Adaptación y validación del Voice Handicap Index y su versión abreviada al español rioplatense de Argentina

Objetivo: Este estudio tuvo como objetivo principal validar el Voice Handicap Index (VHI) y su versión abreviada (VHI-10) adaptados al español rioplatense de Argentina, con objetivos específicos centrados en evaluar su fiabilidad y validez. Metodología: La adaptación cultural incluyó técnicas de traducción directa, síntesis y retrotraducción, evaluación de la equivalencia semántica y aplicación a un grupo piloto. Para la validación se evaluó la fiabilidad de ambos índices adaptados mediante la consistencia interna (coeficiente alfa de Cronbach) y la estabilidad test-retest (prueba de Bland-Altman, CCI y r de Spearman). Además, se examinó la validez de criterio y de constructo. 213 sujetos participaron en la validación del índice adaptado de 30 ítems (123 disfónicos; 90 de control); 113, en la del índice abreviado (63 disfónicos; 50 de control). Resultados: Se constituyó el Índice de Desventaja Vocal (IDV) como la versión adaptada del VHI al español rioplatense de Argentina. Ambos índices demostraron excelente consistencia interna (IDV-30 α = 0,96; IDV-10 α = 0,92) y estabilidad y concordancia (IDV-30 CCI = 0,95; IDV-10 CCI = 0,96). Se halló alta correlación entre los puntajes de ambos índices y la autoevaluación de la severidad de la disfonía de los participantes (r = 0,85). Ambos índices demostraron capacidad de diferenciar entre individuos con disfonía y sujetos sanos (p< 0,001). El análisis factorial reveló tres factores para el IDV-30 y un factor para el IDV-10. Conclusiones: El IDV-30 e IDV-10 presentan grados adecuados de fiabilidad y validez. Ambos pueden ser incluidos en protocolos de valoración de la función vocal por profesionales de Argentina.

Aim: This study aimed to validate the Voice Handicap Index (VHI) and its abbreviated version (VHI-10) adapted into Rioplatense Spanish from Argentina, with specific goals centered on assessing their reliability and validity. Methods: Cultural adaptation involved direct translation, synthesis and back-translation techniques, followed by an assessment of semantic equivalence and application to a pilot group. For the validation process, the reliability of both adapted indices was assessed through measures of internal consistency (Cronbach's alpha coefficient) and test-retest stability (Bland-Altman test, ICC and Spearman's correlation coefficient). Additionally, we conducted analyses to asses criterion and construct validity. 213 subjects participated in the validation of the adapted 30-items index, (123 with dysphonia; 90 from control group); 113, in the abbreviated version (63 with dysphonia; 50 from control group). Results: The "Índice de Desventaja Vocal" (IDV) was established as the adapted version of the VHI into Rioplatense Spanish from Argentina. Both indeces exhibited excellent internal consistency (IDV-30 α = 0,96; IDV-10 α = 0,92) and satisfactory stability and agreement (IDV-30 CCI = 0,95; IDV-10 CCI = 0,96). Regarding validity, a strong correlation was observed between the scores of both indeces and the participant's self-assessment of dysphonia degree (r = 0,85). Both indices effectively differentiated between individuals with dysphonia and healthy subjects (p< 0,001). Factor analysis revealed three factors for the IDV-30 and one factor for the IDV-10. Conclusion: The IDV-30 and IDV-10 demonstrate satisfactory levels of reliability and validity. Both indices can be incorporated into the assessment protocols for evaluating the vocal function by professionals in Argentina.

Dung removal increases under higher dung beetle functional diversity regardless of grazing intensification.

Dung removal by macrofauna such as dung beetles is an important process for nutrient cycling in pasturelands. Intensification of farming practices generally reduces species and functional diversity of terrestrial invertebrates, which may negatively affect ecosystem services. Here, we investigate the effects of cattle-grazing intensification on dung removal by dung beetles in field experiments replicated in 38 pastures around the world. Within each study site, we measured dung removal in pastures managed with low- and high-intensity regimes to assess between-regime differences in dung beetle diversity and dung removal, whilst also considering climate and regional variations. The impacts of intensification were heterogeneous, either diminishing or increasing dung beetle species richness, functional diversity, and dung removal rates. The effects of beetle diversity on dung removal were more variable across sites than within sites. Dung removal increased with species richness across sites, while functional diversity consistently enhanced dung removal within sites, independently of cattle grazing intensity or climate. Our findings indicate that, despite intensified cattle stocking rates, ecosystem services related to decomposition and nutrient cycling can be maintained when a functionally diverse dung beetle community inhabits the human-modified landscape.

Consensus Auditory Perceptual Evaluation of Voice (CAPE-V): revisión sistemática de los métodos utilizados para su adaptación y validación

Introducción: CAPE-V es ampliamente utilizado para evaluación perceptual vocal y ha sido adaptado y validado en múltiples idiomas. A través de un análisis exhaustivo, este estudio buscó avanzar en establecer un estándar en el método utilizado para su adaptación y validación. Objetivo: Revisar los artículos publicados entre 2002 y 2022 que han adaptado y validado CAPE-V a distintos idiomas, para evaluar exhaustivamente la adaptación, metodología y estadísticas utilizadas. Métodos: Se realizó una revisión sistemática utilizando Scopus, Google Scholar y PubMed para identificar artículos que adaptaran y/o validaran CAPE-V entre 2002 y 2022. Se analizó el título y resumen para preseleccionar la muestra. Para evaluar el riesgo de sesgo de los estudios incluidos se analizó de forma crítica el texto completo. Resultados: La búsqueda inicial identificó 568 artículos. Al eliminar duplicados se revisaron 559 y 23 fueron preseleccionados. 12 se incluyeron finalmente y fueron analizados, considerando adaptación, metodología y análisis estadístico. Los resultados revelan que la adaptación y validación de CAPE-V a diversos idiomas es fundamental para garantizar mediciones precisas y confiables en diferentes poblaciones. Análisis y discusión: Existe heterogeneidad en la forma de realizar la adaptación y validación de CAPE-V. No obstante, todos los estudios tuvieron éxito en producir resultados válidos, subrayando la importancia de estos procesos para la práctica clínica. Conclusiones: Las adaptaciones y validaciones de CAPE-V se realizaron heterogéneamente por ausencia de un protocolo estándar. Es necesario generar orientaciones para realizar estos procesos por el aporte de esta escala a la clínica, asegurando calidad y confiabilidad de los resultados.

Introduction: CAPE-V is widely used for vocal perceptual evaluation and has been adapted and validated in multiple languages. Through an exhaustive analysis, this study sought to advance in establishing a standard in the method used in its adaptation and validation. Objective: To review articles published between 2002 and 2022 that have adapted and validated CAPE-V in different languages, to thoroughly evaluate the adaptation, methodology, and statistics used. Methods: A systematic review was conducted using Scopus, Google Scholar and PubMed to identify articles that adapted and/or validated CAPE-V between 2002 and 2022. The title and summary were analyzed to pre-select the sample. To evaluate the risk of bias of the included studies, the full text was critically analyzed. Results: The initial search identified 568 items. When duplicates were removed, 559 were reviewed and 23 were pre-selected. 12 were finally included and analyzed considering adaptation, methodology, and statistical analysis. The results show that the adaptation and validation of CAPE-V to different languages is essential to ensure accurate and reliable measurements in different populations. Analysis and discussion: There is heterogeneity in how CAPE-V is adapted and validated. However, all studies were successful in producing valid results, underlining the importance of these processes for clinical practice. Conclusions: Adaptations and validations of CAPE-V were performed heterogeneously due to the absence of a standard protocol. It is necessary to generate guidelines to perform these processes by providing this scale to the clinic, ensuring quality and reliability of results.

Global Research Trends on the Utilization of Nopal (Opuntia Sp) Cladodes as a Functional Ingredient for Industrial Use.

Although nopal cladodes are a valuable bioactive compound source, they have historically been underused. This review draws a parallel between quantitative and qualitative data from the most outstanding scientific research concerning nopal cladodes in the last five years by implementing a bibliometric analysis. Italy, Mexico, Brazil, and Morocco accounted for approximately 55% of the 111 articles selected for this review. Nopal cladodes are a great source of nourishing ingredients such as mucilage, pectin, insoluble fibers, minerals, ascorbic acid, and bioactive compounds such as carotenoids (e.g., ß-carotene, lutein, and cryptoxanthin), flavonoids (e.g., isorhamnetin, quercetin, rutin, and catechin), phytosterols (e.g., ß-sitosterol and ß-campesterol). Additionally, they offer technological benefits as a food ingredient, allied to good sensory acceptability. The findings suggest that medium-aged cladodes (20 days) have the highest concentration of soluble fiber, protein, and bioactive compounds, rendering them the optimal maturity stage for consumption and processing. Therefore, nopal cladodes can be exploited for several industries, including biotechnology, cosmetics, and pharmaceuticals, and they have attracted attention as a promising ingredient for the food industry in the concept of the next generation of innovative and functional vegetable foods.

Antiviral Activity of Zinc Oxide Nanoparticles against SARS-CoV-2.

The highly contagious SARS-CoV-2 virus is primarily transmitted through respiratory droplets, aerosols, and contaminated surfaces. In addition to antiviral drugs, the decontamination of surfaces and personal protective equipment (PPE) is crucial to mitigate the spread of infection. Conventional approaches, including ultraviolet radiation, vaporized hydrogen peroxide, heat and liquid chemicals, can damage materials or lack comprehensive, effective disinfection. Consequently, alternative material-compatible and sustainable methods, such as nanomaterial coatings, are needed. Therefore, the antiviral activity of two novel zinc-oxide nanoparticles (ZnO-NP) against SARS-CoV-2 was investigated in vitro. Each nanoparticle was produced by applying highly efficient "green" synthesis techniques, which are free of fossil derivatives and use nitrate, chlorate and sulfonate salts as starting materials and whey as chelating agents. The two "green" nanomaterials differ in size distribution, with ZnO-NP-45 consisting of particles ranging from 30 nm to 60 nm and ZnO-NP-76 from 60 nm to 92 nm. Human lung epithelial cells (Calu-3) were infected with SARS-CoV-2, pre-treated in suspensions with increasing ZnO-NP concentrations up to 20 mg/mL. Both "green" materials were compared to commercially available ZnO-NP as a reference. While all three materials were active against both virus variants at concentrations of 10-20 mg/mL, ZnO-NP-45 was found to be more active than ZnO-NP-76 and the reference material, resulting in the inactivation of the Delta and Omicron SARS-CoV-2 variants by a factor of more than 106. This effect could be due to its greater total reactive surface, as evidenced by transmission electron microscopy and dynamic light scattering. Higher variations in virus inactivation were found for the latter two nanomaterials, ZnO-NP-76 and ZnO-NP-ref, which putatively may be due to secondary infections upon incomplete inactivation inside infected cells caused by insufficient NP loading of the virions. Taken together, inactivation with 20 mg/mL ZnO-NP-45 seems to have the greatest effect on both SARS-CoV-2 variants tested. Prospective ZnO-NP applications include an antiviral coating of filters or PPE to enhance user protection.

Cassava starch films for food packaging: Trends over the last decade and future research.

Cassava starch is one of the most available and cost-effective biopolymers. This work aimed to apply a bibliometric methodology to identify the most impactful scientific data on cassava starch and its residues for food packaging in the last ten years. As a result, an increasing interest in this subject has been observed, mainly in the past five years. Among the 85 selected scientific publications, Brazil and China have been leading the research on starch-based films, accounting for 39 % of the total. The International Journal of Biological Macromolecules was the main scientific source of information. Besides cassava starch, 41.18 % of these studies added other biopolymers, 5.88 % added synthetic polymers, and 4.71 % added a combination of both. Studies analyzed suggested that different modifications in starch can improve films' mechanical and barrier properties. In addition, 52.94 % of articles evaluated the film's bioactivity. Still, only 37.65 % assessed the performance of those films as food packaging, suggesting that more studies should be conducted on assessing the potential of these alternative packages. Future research should consider scale-up methods for film production, including cost analysis, assessment life cycle, and the impact on the safety and quality of a broader range of foods.

Lanthanide (Eu, Tb, La)-Doped ZnO Nanoparticles Synthesized Using Whey as an Eco-Friendly Chelating Agent.

Strategies for production and use of nanomaterials have rapidly moved towards safety and sustainability. Beyond these requirements, the novel routes must prove to be able to preserve and even improve the performance of the resulting nanomaterials. Increasing demand of high-performance nanomaterials is mostly related to electronic components, solar energy harvesting devices, pharmaceutical industries, biosensors, and photocatalysis. Among nanomaterials, Zinc oxide (ZnO) is of special interest, mainly due to its environmental compatibility and vast myriad of possibilities related to the tuning and the enhancement of ZnO properties. Doping plays a crucial role in this scenario. In this work we report and discuss the properties of undoped ZnO as well as lanthanide (Eu, Tb, and La)-doped ZnO nanoparticles obtained by using whey, a by-product of milk processing, as a chelating agent, without using citrate nor any other chelators. The route showed to be very effective and feasible for the affordable large-scale production of both pristine and doped ZnO nanoparticles in powder form.

Aplicativo móvel para avaliação de complicações cardíacas: tecnologia assistencial / Mobile app for assessment of cardiac complications: assistive technology

Resumo: Introdução: A cirurgia cardíaca é uma estratégia de tratamento para prolongar a vida das pessoas proporcionando aumento da expectativa de vida. A Identificação de complicações cardíacas dos pacientes no período do pós-operatório é essencial para o sucesso dessa modalidade terapêutica. Objetivo: desenvolver uma tecnologia assistencial para avaliação de complicações em diferentes órgãos e sistemas do organismo após a cirurgia cardíaca. Método: Pesquisa Metodológica de Desenvolvimento Tecnológico, desenvolvida em três fases: Análise do tema; Desenho e Desenvolvimento do Aplicativo; Avaliação do aplicativo em seis etapas. A pesquisa foi desenvolvida na Unidade de Terapia Intensiva de um hospital no município de Jaraguá do Sul. O grupo de participantes foi composto por cinco profissionais da equipe multiprofissional da Unidade de Terapia Intensiva. Os dados foram obtidos por meio de um instrumento de coleta de dados elaborado pela pesquisadora e registros diários do prontuário eletrônico. Resultados: aplicativo móvel denominado CardioTIS®, com oito interfaces: Tela de apresentação; Cadastro/Login do usuário; Registro ou busca do paciente; Identificação; Anamnese; Dados vitais; Dados clínicos e Avaliação; o qual aborda a avaliação e identificação prévia de sinais clínicos, apresenta alertas de sinais clínicos alterados, que antecedem as complicações da cirurgia cardíaca no período do pós-operatório de pacientes assistidos na Unidade de Terapia Intensiva e orientações para a equipe. Conclusão: O aplicativo CardioTIS®, segundo a avaliação dos juízes, nos quesitos, funcionalidade, confiabilidade, usabilidade e eficiência, alcançou índices que demostram ser viável a identificação de sinais e sintomas que apontam as prováveis complicações pós-operatória de cirurgia cardíaca e subsidia a equipe para a tomada da melhor decisão, avisando intercorrências no restabelecimento do paciente, colabora para a segurança do paciente, da equipe e sucesso no tratamento cirúrgico.

Abstract: Introduction: Cardiac surgery is a treatment strategy to lengthen people's lives by providing life expectancy. identifying cardiac complications in patients in the postoperative period is crucial for the success of this therapeutic modality. Objective: to develop an assistant technology to evaluate complications after cardiac surgery. Method: Technological Development Methodological Research, developed in three phases: Theme analysis; Application design and development; Application evaluation and six steps. The research site was a large hospital in the city of Jaraguá do Sul, specifically in the Intensive Care Unit. The group of participants consisted of the multidisciplinary team of the Intensive Care Unit. Data collection was carried out through an instrument developed by the researcher and daily records from the electronic medical record. Results: a mobile application named CardioTIS®, with eight interfaces: Introduction screen; User Registration/Login; Patient registration or search; Identification; Anamnesis; Vital signs; Clinical data and evaluation, which addresses prior identification and evaluation of clinical signs, alarms with guidance, that precede cardiac surgery complications in the postoperative period of patients assisted in the Intensive Care Unit. Conclusion: The CardioTIS® app, according to the judges' assessment, regarding functionality, reliability, usability, and efficiency, reached rates that show the possibility of enabling the identification of signs and symptoms that point to possible postoperative complications of cardiac surgery and subsidizes the team with the best decision-making, warning of complications in the patient's recovery, contributing to the safety of the patient and team safety and surgical treatment achievement.

Nopal cladode as a novel reinforcing and antioxidant agent for starch-based films: A comparison with lignin and propolis extract.

The potential use of nopal cladode flour (NC) as reinforcing/bioactive agent in cassava starch-based films was evaluated and compared with the use of propolis extract or lignin, which are commonly used for these purposes. Cassava starch-based films containing untreated NC (S-NC), NC treated at pH 12 (S-NC12), aqueous propolis extract at two different concentrations (SP1 or SP2), or lignin (S-L) were produced by the casting technique; glycerol was used as plasticizer. NC12 and NC affected the mechanical properties of the cassava starch-based film similarly as compared to propolis extract and lignin. Moreover, NC and NC12 had different performance as reinforcing and antioxidant agent in cassava starch-based film. Thus, S-NC12 film was more elongable (28.5 ± 6.5%), more hydrophobic (contact angle: 70.8° ± 0.1), less permeable to water vapor (0.8 ± 0.0 × 10-10 g·m-1·s-1·Pa-1) and had better antioxidant activity by ABTS•+ (44.70 ± 0.3 µM Trolox·g-1 of film) than the S-NC film. SEM and TGA analysis of films showed that NC12 was better incorporated into the cassava starch matrix than NC, lignin and propolis extract. Overall, nopal cladode flour has potential use in the production of active biodegradable packaging for the food preservation with high oxidation rate.

Minerals in Pregnancy and Their Impact on Child Growth and Development.

During pregnancy, women undergo metabolic and physiological changes, and their needs are higher, to maintain growth and development of the fetus. If the nutritional status of the expectant mother is not satisfactory, some maternal and neonatal complications can occur. In the second and third trimester of pregnancy, there is a reserve of nutrients in the fetus that can be utilized after birth; thereby, children present an accelerated growth in the first years of life, which is a proven response to the available nutrition pattern. However, if such a pattern is insufficient, there will be deficits during development, including brain function. Therefore, despite many recent published works about gestational nutrition, uncertainties still remain on the mechanisms of absorption, distribution, and excretion of micronutrients. Further elucidation is needed to better understand the impacts caused either by deficiency or excess of some micronutrients. Thus, to illustrate the contributions of minerals during prenatal development and in children, iodine, selenium, iron, zinc, calcium, and magnesium were selected. Our study sought to review the consequences related to gestational deficiency of the referred minerals and their impact on growth and development in children born from mothers with such deficiencies.

Assessment of Human Health Risks Posed by Nano-and Microplastics Is Currently Not Feasible.

The exposure of humans to nano-and microplastic particles (NMPs) is an issue recognized as a potential health hazard by scientists, authorities, politics, non-governmental organizations and the general public. The concentration of NMPs in the environment is increasing concomitantly with global plastic production and the usage of plastic materials. NMPs are detectable in numerous aquatic organisms and also in human samples, therefore necessitating a risk assessment of NMPs for human health. So far, a comprehensive risk assessment of NMPs is hampered by limited availability of appropriate reference materials, analytical obstacles and a lack of definitions and standardized study designs. Most studies conducted so far used polystyrene (PS) spheres as a matter of availability, although this polymer type accounts for only about 7% of total plastic production. Differently sized particles, different concentration and incubation times, and various biological models have been used, yielding hardly comparable data sets. Crucial physico-chemical properties of NMPs such as surface (charge, polarity, chemical reactivity), supplemented additives and adsorbed chemicals have been widely excluded from studies, although in particular the surface of NMPs determines the interaction with cellular membranes. In this manuscript we give an overview about the critical parameters which should be considered when performing risk assessments of NMPs, including novel reference materials, taking into account surface modifications (e.g., reflecting weathering processes), and the possible role of NMPs as a substrate and/or carrier for (pathogenic) microbes. Moreover, we make suggestions for biological model systems to evaluate immediate toxicity, long-term effects and the potential of NMPs to cross biological barriers. We are convinced that standardized reference materials and experimental parameters along with technical innovations in (nano)-particle sampling and analytics are a prerequisite for the successful realization of conclusive human health risk assessments of NMPs.

Novel nitroimidazole derivatives evaluated for their trypanocidal, cytotoxic, and genotoxic activities.

The current treatment of Chagas disease is based on the use of two drugs, nifurtimox (Nfx) and benznidazole (Bnz), both of which present limited efficacy in the chronic stage of the disease and toxic side effects. Thus, the discovery of novel compounds is urgently required. Herein, we report the successful synthesis of 4-nitroimidazole analogs of Bnz via nucleophilic aromatic substitution or cycloaddition reactions. The analogs were biologically evaluated, and compound 4 (4-cyclopropyl-1-(1-methyl-4-nitro-1H-imidazole-5-yl)-1H-1,2,3-triazole) was identified as the most potent against both the trypomastigote (IC50 = 5.4 µM) and amastigote (IC50 = 12.0 µM) forms of T. cruzi, showing activity in the same range as Bnz (IC50 = 8.8 and 8.7 µM, respectively). The cytotoxic and genotoxic activities of compounds 5, 4 and 11 were assessed. These three compounds were cytotoxic and genotoxic to RAW and HepG2 cells and mutagenic to Salmonella enterica strains. However, 4 exhibited toxic effects only at concentrations higher than those needed for trypanocidal activity. Molecular docking of 4 showed the importance of the size and π-π interactions between the nitroimidazole and the cofactor (flavin mononucleotide) of T.cruzi-nitroreductase (TcNTR). Moreover, the residues His503 and Tyr545 are relevant for binding to TcNTR. Our design strategy was capable of generating novel and active Bnz analogs.

Experiência materna no cuidado do filho com paralisia cerebral: análise fundamentada na teoria de Roy / Maternal experience in the care of the child with cerebral palsy: analysis based on Roy theory / Experiencia materna en el cuidado del hijo con parálisis cerebral: análisis fundamentado en la teoría de Roy

O objetivo do estudo aqui apresentado é descrever a experiência das mães de crianças com paralisia cerebral à luz da teoria de adaptação de Roy e identificar estímulos significativos assim como mecanismos de enfrentamentos no cuidado. Trata-se de um estudo descritivo com abordagem qualitativa. Participaram oito mães de filhos com paralisia cerebral, cadastrados na Associação de Pais e Amigos dos Excepcionais - Apae de um município do Ceará. A pesquisa ocorreu entre abril e maio de 2014 e utilizou-se para coleta de dados a entrevista semiestruturada e, para análise, o método de categorização temática. A teoria permitiu reconhecer que as mães podem desencadear respostas positivas ou negativas mediante estímulos que giram em torno do diagnóstico do filho: impacto, sentimentos, contexto intrafamiliar envolvido no cuidado, conflitos, enfrentamentos. Concluiu-se que o enfermeiro deve objetivar, em seu trabalho, respostas adaptativas, minimizando as respostas ineficientes, de modo a obter a melhoria do cuidado prestado a mãe-criança e fortalecer o vínculo afetivo entre ambos

This study aims to describe the experience of the mothers of children with cerebral palsy in the light of Royl adaptation model, and to identify significant stimuli and adaptive mechanisms to cope with the situation in care. It is a descriptive study with qualitative approach. The subjects were eight mothers of children with cerebral palsy, enrolled in the APAE ­ Associação de Pais e Amigos dos Excepcionais [Association of Parents and Friends of the People with Special Needs] of a municipality in Ceará, Brazil. The research took place from April to May 2014 and used to data collection a semi-structured interview and for analysis the method of thematic categorization. The Roy theory allowed to recognize that mothers can trigger positive or negative responses through stimuli related to the nursing diagnosis of the child illness: impact, feelings, intrafamily context involved in care, conflicts, confrontations. We had concluded that the nursing should aim for adaptive responses in her work, minimizing the ineffective responses so to obtain the improvement of the mother-child care and to strengthen the affective bond between both

El objetivo de este estudio es describir la experiencia de las madres de niños com parálisis cerebral a la luz de la teoría de adaptación de Roy y identificar estímulos significativos y mecanismos de enfrentamiento en el cuidado. Él es un estudio descriptivo con enfoque cualitativo. Los sujetos fueron ocho madres de hijos con parálisis cerebral, registrados en la APAE ­ Associação de Pais e Amigos dos Excepcionais [Asociación de Padres y Amigos de los Excepcionales] de un municipio de Ceará, Brasil. La investigación ocurrió de abril a mayo de 2014 y utilizó para la recolección de datos una entrevista semiestructurada y para el análisis el método de la categorización temática. Esa teoría permitió reconocer que las madres pueden desencadenar respuestas positivas o negativas mediante estímulos relacionados al diagnóstico de la dolencia del hijo: impacto, sentimientos, contexto intrafamiliar envuelto en el cuidado, conflictos, enfrentamientos. Concluimos que el enfermero debe objetivar en su trabajo, respuestas adaptativas, minimizando las respuestas ineficientes, para obtener la mejora del cuidado prestado a la madre-niño y fortalecer el vínculo afectivo entre ambos

Entrenamiento audioperceptual (EAP) en disfonías. Resultados preliminares del primer taller experimental / The audioperceptual evaluation for dysphonias. Preliminary results of an experimental workshop / Formação audio perceptiva (EAP) de disfunção. Resultados preliminares do primeiro workshop experimental

INTRODUCCIÓN: El entrenamiento audioperceptual (EAP) puede ser utilizado como elemento diagnóstico laringológico para dirigir el estudio del paciente disfónico a un nivel de mayor complejidad en caso de discrepancia entre lo percibido perceptualmente y el estudio endoscópico realizado. El objetivo de este trabajo fue valorar el EAP como herramienta complementaria diagnóstica en la percepción y calificación individual de la voz del paciente con patología vocal en un grupo selecto de profesionales fonoaudiólogos y otorrinolaringólogos...

INTRODUCTION: Audioperceptual training (APT) can be used as a laryngological diagnostic element to direct the study of the dysphonic patient to a more complex level in the event of a discrepancy between what is perceptually perceived and the endoscopic study performed. The objective of this work was to evaluate the APT as a complementary diagnostic tool in the perception and individual qualification of the voice of the patient with vocal pathology in a select group of professional phonoaudiologists and otolaryngologists…

INTRODUÇÃO: O treinamento Audioperceptual (EAP) pode ser usado como um elemento de diagnóstico laringológico para direcionar o estudo do paciente disfônico para um nível mais complexo em caso de discrepância entre o que é percebido perceptualmente e o estudo endoscópico realizado. O objetivo deste trabalho foi avaliar o EAP como ferramenta de diagnóstico complementar na percepção e qualificação individual da voz do paciente com patologia vocal em um seleto grupo de fonoaudiólogos e otorrinolaringologistas profissionais...

Alterations in the extracellular catabolism of nucleotides are involved in the antiproliferative effect of quercetin in human bladder cancer T24 cells.

Bladder cancer is the most prevalent tumor in the genitourinary tract and the current treatments are not efficient to prevent recurrence and progression of tumor cases. Studies have revealed evidence of the involvement of the purinergic system in bladder tumorigenesis, particularly ecto-5'-NT/CD73, the enzyme responsible for AMP hydrolysis. Quercetin (3,3',4',5,7-pentahydroxyflavone) is a plant-derived flavonoid that has been shown to exert a broad range of pharmacologic properties, including potential anticancer activity. Here, we investigated the quercetin effect on the E-NTPDases and ecto-5'-nucleotidase/CD73, which catalyzes the introversion of the extracellular purine nucleotides in T24 human bladder cancer cells. The results showed that this flavonoid was able to increase ADP hydrolysis and inhibit the ecto-5'-nucleotidase/CD73 activity, with no effect on protein expression. The treatment with APCP (α,ß-methyleneadenosine-5'-diphosphate), another ecto-5'-NT/CD73 inhibitor, led to a significant reduction in cell proliferation. In addition, we showed that AMP, which can be accumulating by enzyme inhibition, had an antiproliferative effect on T24 cells, which was enhanced when its hydrolysis was inhibited by APCP treatment. Otherwise, adenosine did not cause any significant effect on cell proliferation and the quercetin effects were not altered by the simultaneous presence of adenosine. Taken together, the results suggest that the antiproliferative effect of quercetin on tumor cells may occur, at least in part, via alterations in the extracellular catabolism of nucleotides, that could be by AMP accumulation, or could be due to blocked adenosine receptors by this flavonoid, supporting the potential use of quercetin in bladder cancer treatment.

First records of Glyphepomis adroguensis (Hemiptera, Pentatomidae) and its parasitoid, Telenomus podisi (Hymenoptera, Platygastridae), on irrigated rice fields in Rio Grande do Sul, Brazil / Primeiros registros de Glyphepomis adroguensis (Hemiptera, Pentatomidae) e seu parasitoide, Telenomus podisi (Hymenoptera, Platygastridae), em arroz irrigado no Rio Grande do Sul

First records of Glyphepomis adroguensis (Hemiptera, Pentatomidae) and its parasitoid, Telenomus podisi (Hymenoptera, Platygastridae), on rice fields in Rio Grande do Sul, Brazil. Eggs, nymphs, and adults of Glyphepomis adroguensis Berg, 1891 (Hemiptera, Pentatomidae) were observed for the first time on rice fields (Oryza sativa L.) in Charqueadas (29º59'S, 51º31'W) and Eldorado do Sul (30º02'S, 51º23'W) of Rio Grande do Sul state, Brazil. Telenomus podisi Ashmead, 1893 (Hymenoptera, Platygastridae) was found in G. adroguensis eggs.

Primeiros registros de Glyphepomis adroguensis (Hemiptera, Pentatomidae) e seu parasitoide, Telenomus podisi (Hymenoptera, Platygastridae), em arroz irrigado no Rio Grande do Sul. Ovos, ninfas e adultos de Glyphepomis adroguensis Berg, 1891 (Hemiptera, Pentatomidae) foram observados pela primeira vez no Rio Grande do Sul, Brasil, em lavouras de arroz irrigado (Oryza sativa L.) em Charqueadas (29º59'S, 51º31'W) e Eldorado do Sul (30º02'S, 51º23'W). Telenomus podisi Ashmead, 1893 (Hymenoptera, Platygastridae) foi encontrado em ovos de G. adroguensis.

Glicemical analysis of human blood serum using FT-Raman: a new approach.

BACKGROUND DATA: Fourier transform Raman spectroscopy (FT-Raman) is a noninvasive diagnostic tool largely applied to the analysis of biological fluids and tissues. METHODS: We examined the variation of carbohydrate concentration in human blood during 180 min relative to 29 spectra of patients split into four categories: hypoglycemic, healthy, threshold, and diabetic. The main bands monitored were placed in 960, 1030, 1091, 1128, and 1205 cm(-1). These bands were respectively attributed to C-O, C-C (stretching), C-O-H and C-O-C of carbohydrates. In this study, the Raman scattering signal of the all the blood samples was collected during 360 sec. The calculated correlation coefficient (R) between the concentration of carbohydrates and the Raman intensity was 0.923. CONCLUSIONS: The obtained results are reasonable according to classical biochemical analysis. Our proposed FT-Raman-based method was shown to be suitable for the monitoring of carbohydrate concentration in human blood, and presented some advantages over classical biochemical methods, such as real-time analysis, required small sample volume, and was nondestructive, and the samples did not need any previous treatment.

Distintos enfoques sobre esteroides anabolizantes: riscos à saúde e hipermasculinidade / Distinct approaches towards anabolic steroids: risks to health and hypermasculinity / Distintos enfoques sobre los esteroides anabólicos: riesgos para la salud e hipermasculinidad

Abordam-se dois enfoques sobre o uso de esteroides anabólicos androgênicos (EAA): o primeiro encontra-se na literatura biomédica, centrada em seus efeitos na saúde de homens jovens; o segundo refere-se aos próprios usuários, focalizando os aspectos socioculturais do consumo. Foi utilizada metodologia qualitativa a partir da análise de dois tipos de material: (i) artigos da área biomédica; (ii) material de pesquisas realizadas entre 2001 e 2004, incluindo etnografia e entrevistas semiestruturadas com 19 homens praticantes de jiu-jítsu no Rio de Janeiro. Os resultados indicam um descompasso entre o enfoque biomédico, que condena o uso considerado não terapêutico de EAA e as representações e práticas dos usuários que recorrem a essas substâncias com o objetivo de aprimoramento da força e da musculosidade. Conclui-se que a relação entre o consumo de substâncias anabolizantes e a construção social da masculinidade precisa ser mais estudada no âmbito da saúde pública.

Two approaches towards anabolic androgenic steroid (AAS) use are dealt with: the first is found in the biomedical literature, centered on the effects on young men's health; the second refers to the users themselves, focusing on the sociocultural aspects of consumption. Qualitative methodology was used, based on analysis of two types of material: (i) articles from the biomedical field; (ii) material from studied conducted between 2001 and 2004, including ethnography and semi-structured interviews with nineteen male jiu-jitsu fighters in Rio de Janeiro. The results indicate that there is a mismatch between the biomedical approach, which condemns the non-therapeutic use of AAS, and the representations and practices of users who resort to these substances with the goal of improving strength and muscularity. It is concluded that the relationship between anabolic substance consumption and the social construction of masculinity needs to be further studied within the context of public health.

Esta investigación aborda dos enfoques sobre el uso de esteroides anabólicos androgénicos (EAA): uno encontrado en la literatura biomédica, que estudia sus efectos en la salud de varones jóvenes; el otro se refiere a los propios usuarios, señalando los aspectos socioculturales del consumo. La metodología ha sido cualitativa, basada en el análisis de dos tipos de materiales: artículos del área biomédica y materiales de estudios anteriores (2001-2004), incluyendo etnografía y entrevistas semiestructuradas con 19 jóvenes practicantes de jiu-jitsu en Río de Janeiro. Los resultados apuntan a una divergencia entre el enfoque biomédico, que condena el uso no terapéutico de EAA, y las representaciones y prácticas de los usuarios que recurren a estas substancias con el objetivo de desarrollar la fuerza y la musculatura. El vínculo entre el consumo de substancias anabolizantes y la construcción social de la masculinidad necesita un estudio más profundizado en el ámbito de la salud pública.

Determinación de zonas de riesgo al agua arsenical y prevalencia de hacre en Villa Belgrano, Tucumán, Argentina / Determination of zones exposed to Arsenical Water and CERHA prevalence in Villa Belgrano, Tucumán, Argentina

INTRODUCCION: En Tucumán la concentración de arsénico en agua fluctúa entre 0,1 y 0,01 ppm. El sistema de salud carece de registro de casos. OBJETIVOS: Elaborar un mapa de riesgo ambiental, estimar la prevalencia de hidroarsenicismo crónico regional endémico (HACRE) y explorar la relación entre la concentración de arsénico en agua y las manifestaciones clínicas en Villa Belgrano, Tucumán. METODOS: estudio transversal. Se analizaron todas las fuentes de agua y se seleccionaron aleatoriamente 119 personas adultas. Para la definición de caso de HACRE se utilizó un criterio diferente al de la Organización Mundial de la Salud (OMS) en concentración de arsénico en agua, orina y cabello y tiempo de consumo, siendo éste más sensible en el valor de corte de arsénico en el agua. Se consideró caso definido a quien había consumido agua con un nivel de arsénico mayor a 0,01 partes por millón(ppm) por más de 5 años y presentado más de 3 ug/g en cabello y/o más de 40 ug/g en orina, con clínica compatible. RESULTADOS: El 75% de las fuentes de agua tenían valores mayores a 0,01 ppm de arsénico. Con los criterios de la OMS, se registraron 51 casos probables y no hubo casos definidos. Según los criterios del estudio, hubo 3 casos definidos y una prevalencia del 2,6%. No se halló una asociación entre lesiones no cutáneas o cutáneas graves y valores mayores a 0,01 ppm. CONCLUSIONES: La prevalencia estimada según los criterios del estudio tuvo valores similares a los establecidos en la bibliografía bajo las condiciones en cuestión, aunque con otros criterios no comparables.(AU)

INTRODUCTION: In Tucumán, the arsenic concentration in water fluctuates between 0,1 and 0,01 ppm.The provincial health system does not have any record of cases. OBJECTIVES: To identify and create a map of environmental risk, estimating also chronic endemic regional hydroarsenicism(CERHA) prevalence in Villa Belgrano and exploring the relation between arsenic concentration in water and clinical manifestations. METHODS: A cross-sectional study was performed. All water sources were analyzed and 119 adults were randomly selected. The definition of case considered the own criteria, which differed from the ones of World Health Organization (WHO) regarding arsenic concentration in water, urine and hair as well as time of consumption. The study criteria is most sensible in arsenic water concentration. Those who had consumed water with more than 0.01 parts per million (ppm) for over 5 years and presented more than 3 ug/g in hair and/or more than 40 ug/gin urine with compatible clinical signs were considered as cases. RESULTS: 75 % of water sources had arsenic levels higher than 0.01 ppm. According to the WHO criteria, there were 51 probable cases and there was not any defined case. According to the study criteria, there were 3 defined cases with a prevalence of 2.6%. The study did not find an association between withnon-cutaneous or serious cutaneous injuries and arsenic levelin water higher than 0.01 ppm. CONCLUSIONS: The prevalence estimated according to the study criteria was similar to the one established in the bibliography under the same conditions, though with other criteria which are not comparable.(AU)

Determinación de zonas de riesgo al agua arsenical y prevalencia de hacre en Villa Belgrano, Tucumán, Argentina / Determination of zones exposed to Arsenical Water and CERHA prevalence in Villa Belgrano, Tucumán, Argentina

INTRODUCCION: En Tucumán la concentración de arsénico en agua fluctúa entre 0,1 y 0,01 ppm. El sistema de salud carece de registro de casos. OBJETIVOS: Elaborar un mapa de riesgo ambiental, estimar la prevalencia de hidroarsenicismo crónico regional endémico (HACRE) y explorar la relación entre la concentración de arsénico en agua y las manifestaciones clínicas en Villa Belgrano, Tucumán. METODOS: estudio transversal. Se analizaron todas las fuentes de agua y se seleccionaron aleatoriamente 119 personas adultas. Para la definición de caso de HACRE se utilizó un criterio diferente al de la Organización Mundial de la Salud (OMS) en concentración de arsénico en agua, orina y cabello y tiempo de consumo, siendo éste más sensible en el valor de corte de arsénico en el agua. Se consideró caso definido a quien había consumido agua con un nivel de arsénico mayor a 0,01 partes por millón(ppm) por más de 5 años y presentado más de 3 ug/g en cabello y/o más de 40 ug/g en orina, con clínica compatible. RESULTADOS: El 75% de las fuentes de agua tenían valores mayores a 0,01 ppm de arsénico. Con los criterios de la OMS, se registraron 51 casos probables y no hubo casos definidos. Según los criterios del estudio, hubo 3 casos definidos y una prevalencia del 2,6%. No se halló una asociación entre lesiones no cutáneas o cutáneas graves y valores mayores a 0,01 ppm. CONCLUSIONES: La prevalencia estimada según los criterios del estudio tuvo valores similares a los establecidos en la bibliografía bajo las condiciones en cuestión, aunque con otros criterios no comparables.(AU)

INTRODUCTION: In Tucumán, the arsenic concentration in water fluctuates between 0,1 and 0,01 ppm.The provincial health system does not have any record of cases. OBJECTIVES: To identify and create a map of environmental risk, estimating also chronic endemic regional hydroarsenicism(CERHA) prevalence in Villa Belgrano and exploring the relation between arsenic concentration in water and clinical manifestations. METHODS: A cross-sectional study was performed. All water sources were analyzed and 119 adults were randomly selected. The definition of case considered the own criteria, which differed from the ones of World Health Organization (WHO) regarding arsenic concentration in water, urine and hair as well as time of consumption. The study criteria is most sensible in arsenic water concentration. Those who had consumed water with more than 0.01 parts per million (ppm) for over 5 years and presented more than 3 ug/g in hair and/or more than 40 ug/gin urine with compatible clinical signs were considered as cases. RESULTS: 75 % of water sources had arsenic levels higher than 0.01 ppm. According to the WHO criteria, there were 51 probable cases and there was not any defined case. According to the study criteria, there were 3 defined cases with a prevalence of 2.6%. The study did not find an association between withnon-cutaneous or serious cutaneous injuries and arsenic levelin water higher than 0.01 ppm. CONCLUSIONS: The prevalence estimated according to the study criteria was similar to the one established in the bibliography under the same conditions, though with other criteria which are not comparable.(AU)